Biodiversity
& Evolution

Subtopic 3.1 — New Syllabus (First Assessment 2026)
Standard Level + Higher Level

9 SL Points
3.1.1 – 3.1.9
10 HL Extensions
3.1.10 – 3.1.19

What You Need to Know

This subtopic defines biodiversity at multiple levels, explains how evolutionary processes generate and maintain it, introduces quantitative measures, and examines the role of human activity in both reducing and reshaping biodiversity across geological time.

A. Biodiversity & Its Importance
Levels of diversity, resilience, ecosystem complexity
B. Evolution & Speciation
Natural selection, mechanisms of evolution, speciation
C. Measuring Biodiversity
Richness, evenness, Simpson's reciprocal index
D. Human Impacts & Deep Time
Artificial selection, geological epochs, mass extinctions

Guiding Questions

Time allocation: minimum 3 hours SL + additional 5–6 hours HL. First subtopic in Topic 3: Biodiversity & Conservation.

Levels of Biodiversity

Key UnderstandingBiodiversity is the total diversity of living systems and it exists at several levels.
Three Levels of Biodiversity: Genetic, Species, and Ecosystem
🧬 Genetic
Diversity
🌿 Species
Diversity
🌍 Habitat / Ecosystem
Diversity
LevelDefinitionExample
Genetic diversityVariation of genes within individuals and populations of a speciesCheetahs: low genetic diversity → vulnerable to disease. Coral reefs: high genetic diversity → resilient to temperature changes
Species diversityNumber of species (richness) and relative abundance (evenness) in a communityTropical rainforest: high richness (50% of world's species on 7% of land). Temperate forest: lower richness but critical for ecosystem services
Habitat diversityVariety of physical environments (forests, wetlands, deserts, reefs) in a regionEstuaries: transition between marine and freshwater → many niches → high biodiversity
The three levels are nested — genetic diversity exists within species, species diversity exists within habitats. Loss at any level weakens the entire system.

Biodiversity & Ecosystem Resilience

Key UnderstandingThe components of diversity contribute to the resilience of ecological systems.

Ecosystem resilience — the ability to absorb disturbance and return to equilibrium — depends on biodiversity at all three levels.

Diversity LevelHow It Builds Resilience
GeneticMore genetic variation → more chance some individuals survive new threats (disease, climate shift)
SpeciesMore species → functional redundancy → if one is lost, others fill its ecological role
HabitatMore habitat types → more refugia during disturbance → faster recovery from fire, flood, drought

Complexity, Feedback & Succession

  • Complex food webs: More connections → more alternative energy pathways → greater stability
  • Negative feedback loops: Diverse ecosystems have more mechanisms to restore balance after disturbance
  • Succession stage: Climax communities (high diversity) are more resilient than pioneer communities (low diversity)
Case study: Wolves reintroduced to Yellowstone → trophic cascade restored ecosystem balance. Sea otters protect kelp forests by controlling sea urchins — a keystone species maintaining resilience.

Evolutionary Processes

Key UnderstandingBiodiversity arises from evolutionary processes.
ProcessMechanismEffect on Diversity
MutationRandom changes in DNA; most neutral or harmful, some beneficialIntroduces new alleles — raw material for evolution
Gene flowMovement of genes between populations via migration & interbreedingIncreases genetic diversity; reduces differences between populations
Genetic driftRandom changes in gene frequencies, especially in small populationsCan reduce diversity; traits may spread or disappear by chance
Natural selectionIndividuals with advantageous traits survive & reproduce morePopulations adapt to environments; drives speciation over time
🎲 Mutation
🔀 Gene flow + Drift
⚔️ Natural selection
🌍 Biodiversity
Key distinction: Genetic drift is random; natural selection is directional. Drift can fix harmful alleles in small populations; selection tends to remove them.

Natural Selection

Key UnderstandingNatural selection is the mechanism driving evolutionary change.

Organisms better adapted to their environment survive, flourish, and reproduce more successfully than those less well adapted — "survival of the fittest."

Peppered Moth Natural Selection Diagram

Case Study: The Peppered Moth (Biston betularia)

  • Pre-Industrial Revolution: Light-colored moths (typica) dominant — camouflaged against lichen-covered bark. Dark form (carbonaria) rare.
  • During Industrial Revolution: Soot darkened tree bark → light moths visible to predators → dark moths favored → population shifted toward carbonaria (industrial melanism)
  • Post–Clean Air Acts: Lichen regrew → bark lightened → light moths regained advantage → typica increased again
  • Key lesson: When the environment changes, the direction of selection reverses — evidence that natural selection is driven by current conditions.

Mechanisms of Natural Selection

Key UnderstandingEvolution by natural selection involves variation, overproduction, competition for limited resources, and differences in adaptation that affect rates of survival and reproduction.
StepWhat HappensConsequence
1. VariationIndividuals differ in traits (size, color, behavior) due to genetic diversityProvides raw material for selection
2. OverproductionSpecies produce more offspring than can surviveLeads to competition for limited resources
3. CompetitionIndividuals with advantageous traits are more likely to surviveDifferential survival based on fitness
4. Differential reproductionSurvivors reproduce more → pass on advantageous traitsFrequency of beneficial alleles increases
5. HeritabilityAdvantageous traits are inherited by offspringPopulation evolves over generations

Example — beetles: In a population of beetles on dark tree bark, dark-colored individuals are better camouflaged from birds → survive more → reproduce more → over generations, the population shifts toward dark coloration.

Exam tip: All five steps are required for a complete explanation of natural selection. Omitting heritability or variation loses marks.

Speciation

Key UnderstandingSpeciation is the generation of new species through evolution.

Speciation occurs when populations of a single species become reproductively isolated, evolve separately, and develop enough genetic divergence that they can no longer interbreed.

Adaptive Radiation in Darwin's Finches

Case Study: Darwin's Finches (Galápagos)

A single immigrant finch species colonized the Galápagos. Geographic isolation on different islands → different food sources → different beak shapes evolved → 13+ distinct species, each adapted to a specific niche. An example of adaptive radiation.

Species Richness & Evenness

Key UnderstandingSpecies diversity in communities is a product of richness and evenness.
ComponentDefinitionWhat It Measures
Species richnessThe total number of different species in an areaHow many species are present (not how common they are)
Species evennessThe relative abundance of each speciesHow evenly individuals are distributed among species

Why Both Matter

  • Two ecosystems can have the same richness (e.g. 20 species) but very different evenness
  • An ecosystem where one species dominates (low evenness) is less resilient than one with balanced abundances
  • High richness + high evenness = maximum diversity = greatest stability and resilience
Exam skill: Given a data table, always calculate or compare both richness and evenness — never assume high richness = high diversity.

Simpson's Reciprocal Index

Key UnderstandingSimpson's reciprocal index is used to provide a quantitative measure of species diversity, allowing different ecosystems to be compared and monitored over time.
Simpson's Diversity Index Formula
D = Σ(n/N)²  |  Reciprocal Index = 1/D

n = number of individuals of each species | N = total number of individuals of all species
Higher 1/D value = greater species diversity

Worked Example

A quadrat sample contains: Species A (40), Species B (35), Species C (25). Total N = 100.

D = (40/100)² + (35/100)² + (25/100)² = 0.16 + 0.1225 + 0.0625 = 0.345

1/D = 1/0.345 ≈ 2.90 → moderate diversity

Skill: You must be able to calculate Simpson's index from a data table. Common error: forgetting to square the fractions (n/N)².

Biodiversity Knowledge & Conservation

Key UnderstandingKnowledge of global and regional biodiversity is needed for the development of effective management strategies to conserve biodiversity.
How Knowledge Is GatheredMethod
Citizen sciencePublic volunteers record species sightings, monitor ecosystems (e.g. bird counts, iNaturalist)
Government & NGOsRemote sensing, field surveys, species monitoring programs (e.g. IUCN Red List assessments)
Indigenous & parabiologistsLocal ecological knowledge combined with scientific methods; trained community members collect data

Conservation Strategies Informed by Knowledge

StrategyHow Knowledge HelpsExample
Protected areasData identifies regions of high ecological value → designated as national parksAmazon reserves; Great Barrier Reef Marine Park
Sustainable managementBiodiversity data informs fishing quotas, forestry practices, water managementMSC-certified sustainable fisheries
Species recovery programsPopulation & genetic data guides captive breeding and habitat restorationCalifornia condor; black-footed ferret recovery
Case study: Gorongosa National Park (Mozambique) — collaboration between government and Carr Foundation uses biodiversity monitoring to guide restoration of a war-damaged ecosystem.

Mutation & Sexual ReproductionHL

Key UnderstandingMutation and sexual reproduction increase genetic diversity.

Mutation

  • Random changes in DNA sequence — occur spontaneously during replication or from mutagens
  • Beneficial: Improve survival/reproduction → spread over generations
  • Neutral / Harmful: Add to gene pool or eliminated by selection
  • Ultimate source of all new genetic variation

Sexual Reproduction

  • Meiosis: Chromosomes exchange material (recombination) → unique gametes
  • Fertilization: Fusion of gametes from 2 parents → unique genotype
  • Ensures every individual (except identical twins) is genetically unique
Populations with high genetic diversity are better equipped to survive environmental change. Low diversity (e.g. agricultural monocultures) → vulnerable to extinction.

Reproductive IsolationHL

Key UnderstandingReproductive isolation can be achieved by geographical separation or, for populations living in the same area, by ecological or behavioural differences.
TypeMechanismExample
Allopatric
(geographic)
Physical barrier (mountain, river, ocean) prevents gene flow between populationsBonobos & chimpanzees: Separated by the Congo River → evolved independently → different social structures and genetics
Sympatric
(same area)
No physical barrier; isolation by ecological niche or behavioral differencesApple maggot fly: Shifted to apple trees → different fruiting times → reproductive isolation within generations
Parapatric
(adjacent)
Continuous range but individuals mate locally; environmental gradient drives divergenceGrass species along a mine toxicity gradient — tolerant and non-tolerant populations diverge

Island Endemism

  • Isolated islands have high endemism (species found nowhere else)
  • Galápagos finches: 13+ species from one ancestor
  • Madagascar lemurs: ~100 species, all endemic — evolved in isolation for ~60 million years

Uneven Distribution of BiodiversityHL

Key UnderstandingBiodiversity is spread unevenly across the planet, and certain areas contain a particularly large proportion of species, especially species that are rare and endangered.
FactorEffect
LatitudeTropical regions (0°–23.5°) have higher species diversity due to stable temperatures and high rainfall
AreaLarger habitats support more species (species-area relationship)
Evolutionary timeTropical regions have been climatically stable for longer → more time for speciation
ProductivityHigher NPP in tropics → more energy → supports more complex food webs

Biodiversity Hotspots

  • Areas with exceptionally high species richness and endemism under significant threat
  • Tropical rainforests: ~50% of world's species on ~7% of Earth's surface
  • Coral reefs: "Rainforests of the sea" — structural complexity supports vast marine diversity
  • 36 recognized hotspots globally (Conservation International criteria)

Human-Driven Evolutionary ChangeHL

Key UnderstandingHuman activities have impacted the selective forces acting on species within ecosystems, resulting in evolutionary change.
Human ActivitySelective PressureEvolutionary Response
Hunting / poachingTargeting individuals with specific traits (e.g. large tusks)Trait reduction or loss in population
UrbanizationNew hazards (traffic), altered food sources, novel habitatsBehavioral adaptation (road-crossing, diet shifts)
AgriculturePesticide use, habitat simplificationPest resistance to pesticides
Climate changeShifting temperature and precipitation patternsRange shifts, phenological changes

Case Study: Tuskless Elephants — Gorongosa, Mozambique

  • During civil war poaching, ~90% of elephants were killed → poachers targeted tusked elephants
  • Tuskless elephants had survival advantage → tuskless gene frequency increased dramatically
  • Demonstrates natural selection driven directly by human selective pressure

Artificial SelectionHL

Key UnderstandingArtificial selection reduces genetic diversity and, consequently, species resilience.

Humans deliberately breed organisms for desired traits — a process called artificial selection. While commercially useful, it drastically narrows the gene pool.

ExampleWhat Was SelectedCost of Reduced Diversity
Cavendish bananaSize, taste, seedlessnessGenetically identical clones — Panama disease (TR4) threatens global production
Commercial wheatHigh yield, uniform growthVulnerable to new fungal strains; relies on chemical inputs
Domestic dog breedsAppearance, temperamentInherited health problems (e.g. breathing in bulldogs, hip dysplasia)
Contrast with natural selection: Natural selection maintains or increases genetic diversity. Artificial selection decreases it by imposing a single, narrow set of criteria.

Deep TimeHL

Key UnderstandingEarth history extends over a period of 4.5 billion years. Processes that occur over an extended timescale have led to the evolution of life on Earth.

The Scale of Earth History

  • 4.5 billion years since Earth formed
  • ~3.8 billion years since first life (prokaryotes)
  • ~540 million years since the Cambrian Explosion (rapid diversification)
  • ~66 million years since the end-Cretaceous mass extinction (dinosaurs extinct)
  • ~300,000 years since Homo sapiens appeared — a fraction of a second in geological time
The processes that built Earth's biodiversity took billions of years. Human impacts are altering biodiversity in decades — orders of magnitude faster than natural evolution can respond.

Geological EpochsHL

Key UnderstandingEarth history is divided up into geological epochs according to the fossil record.
Precambrian
4500–541 Ma
Paleozoic
541–252 Ma
Mesozoic
252–66 Ma
Cenozoic
66 Ma–present

How epochs are defined: Geologists use boundary markers in rock layers — sudden changes in fossil assemblages, chemical signatures (e.g. iridium layer at the K–Pg boundary), or isotopic ratios. The fossil record is the primary archive of Earth's biological history.

Mass Extinctions & SpeciationHL

Key UnderstandingMass extinctions are followed by rapid rates of speciation due to increased niche availability.

The "Big Five" Mass Extinctions

EventWhen (Ma)Species LostLikely Cause
End-Ordovician~443~85%Glaciation, sea-level drop
Late Devonian~372~75%Ocean anoxia, volcanism
End-Permian~252~96%Volcanism (Siberian Traps), climate change
End-Triassic~201~80%Volcanism (CAMP), climate change
End-Cretaceous~66~76%Asteroid impact + Deccan Traps volcanism
💀 Mass extinction
🌿 Empty niches
🆕 Rapid speciation
After the end-Cretaceous extinction: Non-avian dinosaurs disappeared → opened niches for mammals → explosive diversification of mammals. Extinction creates opportunity.

The AnthropoceneHL

Key UnderstandingThe Anthropocene is a proposed geological epoch characterized by rapid environmental change and species extinction due to human activity.

Evidence for the Anthropocene

Evidence TypeWhat It Shows
Extinction rateCurrent extinction rate is 100–1,000× background rate
Nuclear signaturesGlobal fallout from nuclear weapons testing (1945–1963) leaves detectable marker
Plastics & concreteNovel materials now found in sediment layers worldwide
CO₂ levelsAtmospheric CO₂ higher than at any point in 800,000+ years
Land use~75% of ice-free land significantly altered by human activity

Planetary-Scale Human ImpactsHL

Key UnderstandingHuman impacts are having a planetary effect, which will be detectable in the geological record.
Human ImpactGeological SignaturePersistence
CO₂ emissionsCarbon isotope shift (δ¹³C) in sediments; ocean acidification layers100,000+ years
Plastic pollutionPlastic fragments in sedimentary layers worldwideMillions of years
Nuclear falloutRadioactive isotopes (Pu-239) in global sediment layersTens of thousands of years
Species extinctionSudden disappearance of species in fossil recordPermanent
Exam tip: Connect ecological effects (biodiversity loss, climate change) to their geological signatures to show understanding at the systems level.

Essential Vocabulary

TermDefinition
BiodiversityThe total diversity of living systems — genetic, species, and habitat levels
Genetic diversityVariation of genes within individuals and populations of a species
Species diversityThe number of species (richness) and their relative abundance (evenness)
Habitat diversityThe variety of physical environments in a region
ResilienceAn ecosystem's ability to absorb disturbance and recover to equilibrium
Natural selectionIndividuals with advantageous traits survive and reproduce more
SpeciationGeneration of new species through evolution and reproductive isolation
Species richnessThe number of different species in an area
Species evennessThe relative abundance of each species in a community
Simpson's index (1/D)Quantitative measure of species diversity; higher = more diverse
AnthropoceneProposed geological epoch defined by human-dominated environmental change

Key Takeaways & Exam Tips

Must-Know Relationships

  • Genetic → Species → Habitat diversity: Nested levels; loss at any level weakens the system
  • Diversity → Resilience: More species + more genetic variation = more functional redundancy
  • 5 steps of natural selection: Variation, overproduction, competition, differential reproduction, heritability
  • Isolation → Divergence → Reproductive isolation = speciation
  • Richness + Evenness = Species diversity — Simpson's index quantifies both
For 9-mark essays: Always link biodiversity to both ecosystem services and evolutionary processes. Use case studies (peppered moths, Gorongosa elephants, Galápagos finches).

You've covered all 19 syllabus points ✅

3.1.1 – 3.1.9 (SL) + 3.1.10 – 3.1.19 (HL)